Mapeamento genetico rflp, rapd, aflp, microsatelite. The aflp amplified fragment polymorphism technique is used to visualize hundreds of amplified dna restriction fragments simultaneously. Amplified fragmentlength polymorphism aflp is a dna fingerprinting method that employs restriction. Comparison of rapd, rflp, aflp and ssr markers for diversity studies in tropical maize inbred lines antonio a. In the present study three species of heritiera aiton sterculiaceae were characterized using 9 amplified fragment length polymorphism aflp primer combinations and the genetic. Molecular markers in plant systematics and population biology. Comparison of rapd, rflp, aflp and ssr markers for diversity. Amplified fragment length polymorphism aflp is one of the most important molecular marker techniques which is used for fingerprinting and genetic diversity study, aflp based on detecting of dna.
Use of biochemical and molecular markers in genetic diversity studies. A further drawback of aflp technology is perhaps the lack of guarantee of homology between bands of similar molecular. Comparison of rapd, issr, and aflp molecular markers to. Palabras clave aflp diversidad genetica marcador molecular oryza sativa l. In genetic mapping, aflps often cluster at the centromeres and telomeres. Molecular characterization of three heritiera species. They are technically demanding in the laboratory and, especially, in data analysis.
Marcador molecular wikipedia, a enciclopedia livre. In this article we will discuss about the applications of aflp markers in plants. Statistical analysis of amplified fragment length polymorphism data. Three different dnabased techniques, random amplified polymorphic dna rapd, inter simple sequence repeat issr and amplified fragment length polymorphism aflp markers, were used for fingerprinting dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. In this study, rapd assays produced 97 bands, of which 40 were polymorphic 41. Principle of aflp the aflp technique is based on the principle of. Amplified fragment length polymorphism aflp is a novel molecular fingerprinting technique that can be applied to dnas of any source or complexity. The aflp system presents good levels of precision in its genetic estimates and single crosses prediction. Molecular markers in plant systematics and population biology 1. Our results suggest that aflp markers are the best choice for the evaluation of diversity and assessing the genetic relationships between tropical maize inbred lines with high accuracy. The cdnaaflp molecular technique was used to identify genes involved on fruit borer resistance in tomato segregating populations, the evaluated materials of tomato were.
Morfologicos bioquimicos isoenzimas moleculares aflp, microssatelites, snps, etc. As a result of genetic alterations mutations, insertions, deletions, the base composition at. Jun 26, 2019 please use one of the following formats to cite this article in your essay, paper or report. Gene of known function and location gene that allows studying the inheritance of that gene genetic. On the other hand, there are many steps in the aflp detection procedure, which limits its application in marker assisted selec. Amplified fragment length polymorphism an overview. A windows program for the analysis of allozyme and molecular population genetic data. The cdna aflp molecular technique was used to identify genes involved on fruit borer resistance in tomato segregating populations, the evaluated materials of tomato were. The aflp band patterns, or fingerprints, can be used for many. Importance, uses and applications shahid raza 1, muhammad waseem shoaib2 and hira mubeen 1. Sequenciamento da regiao do marcador molecular e busca por genes candidatos. Comparison of rapd, rflp, aflp and ssr markers for. Inter simple sequence repeats issr polymorphism and its aplication in plant.